B cell receptor revision diminishes the autoreactive B cell response after antigen activation in mice
J. Clin. Invest. Ying-Hua Wang, et al. 118:2896 doi:10.1172/JCI35618 [Go to this article.]

Figure 2
Characterization of antigen-reactive and nonreactive B cells. (A) qPCR analysis of transcripts of μ and γ1 chains in antigen-reactive and nonreactive B cells. (B) Sequence (Seq) analysis of VH and Vκ genes expressed by antigen-reactive B cells. Filled circles and triangles represent the number of point mutations in individual VH genes (n = 41) and Vκ genes (n = 26) expressed by Tet⁺B220^lo cells. (C) qPCR analysis of Aid, Blimp1, and Xbp1 in antigen-reactive and nonreactive B cells. Data (mean ± SEM) are representative of 3 independent experiments (A and C). ND, not detected. Mice were immunized with DWEYS-MAP in CFA on day 0 and boosted with DWEYS-MAP in incomplete Freund adjuvant on day 7. Cells were sorted on day 16 (A–C). (D) Flow cytometry analysis of surface CD80 and CD95 on Tet⁺B220^lo (solid line), Tet⁺B220^hi (dotted line), and Tet^–B220^hi (line over shaded area) subsets. Mice were immunized with DWEYS-MAP in CFA on day 0 and boosted with DWEYS-MAP in incomplete Freund adjuvant on days 7 and 56. Spleen and BM cells were prepared 4 days after the second boost for analysis. Representative histogram from 4 to 5 mice was shown.