Palmitate Stimulates the Epithelial Sodium Channel by Elevating Intracellular Calcium, Reactive Oxygen Species, and Phosphoinositide 3‐Kinase Activity
QS Wang, C Liang, N Niu, X Yang… - Oxidative Medicine …, 2018 - Wiley Online Library
Previous studies indicate that the epithelial sodium channel (ENaC) in the kidney is
upregulated in diabetes mellitus. Here, we show that ENaC single‐channel activity in distal
nephron cells was significantly increased by palmitate, a free fatty acid which is elevated in
diabetes mellitus. We also show that palmitate increased intracellular Ca2+ and that after
chelating intracellular Ca2+ with BAPTA‐AM, palmitate failed to affect ENaC activity.
Treatment of the cells with 2‐aminoethoxydiphenyl borate (2‐APB, an inhibitor of IP3 …
upregulated in diabetes mellitus. Here, we show that ENaC single‐channel activity in distal
nephron cells was significantly increased by palmitate, a free fatty acid which is elevated in
diabetes mellitus. We also show that palmitate increased intracellular Ca2+ and that after
chelating intracellular Ca2+ with BAPTA‐AM, palmitate failed to affect ENaC activity.
Treatment of the cells with 2‐aminoethoxydiphenyl borate (2‐APB, an inhibitor of IP3 …
[PDF][PDF] Research Article Palmitate Stimulates the Epithelial Sodium Channel by Elevating Intracellular Calcium, Reactive Oxygen Species, and Phosphoinositide 3 …
QS Wang, C Liang, N Niu, X Yang, X Chen, BL Song… - 2018 - academia.edu
Previous studies indicate that the epithelial sodium channel (ENaC) in the kidney is
upregulated in diabetes mellitus. Here, we show that ENaC single-channel activity in distal
nephron cells was significantly increased by palmitate, a free fatty acid which is elevated in
diabetes mellitus. We also show that palmitate increased intracellular Ca2+ and that after
chelating intracellular Ca2+ with BAPTA-AM, palmitate failed to affect ENaC activity.
Treatment of the cells with 2-aminoethoxydiphenyl borate (2-APB, an inhibitor of IP3 …
upregulated in diabetes mellitus. Here, we show that ENaC single-channel activity in distal
nephron cells was significantly increased by palmitate, a free fatty acid which is elevated in
diabetes mellitus. We also show that palmitate increased intracellular Ca2+ and that after
chelating intracellular Ca2+ with BAPTA-AM, palmitate failed to affect ENaC activity.
Treatment of the cells with 2-aminoethoxydiphenyl borate (2-APB, an inhibitor of IP3 …
