The scarcity of transplant allografts for diseased organs has prompted efforts at tissue
regeneration using seeded scaffolds, an approach hampered by the enormity of cell types
and complex architectures. Our goal was to decellularize intact organs in a manner that
retained the matrix signal for differentiating pluripotent cells. We decellularized intact rat
kidneys in a manner that preserved the intricate architecture and seeded them with
pluripotent murine embryonic stem cells antegrade through the artery or retrograde through …