Molecular markers distinguish bone marrow mesenchymal stem cells from fibroblasts
M Ishii, C Koike, A Igarashi, K Yamanaka, H Pan… - Biochemical and …, 2005 - Elsevier
M Ishii, C Koike, A Igarashi, K Yamanaka, H Pan, Y Higashi, H Kawaguchi, M Sugiyama…
Biochemical and biophysical research communications, 2005•ElsevierTo characterize mesenchymal stem cells (MSC), we compared gene expression profiles in
human bone marrow MSC (11 lines) and human fibroblasts (4 lines) by RT-PCR and real
time PCR. Messenger RNA levels of MHC-DR-α, MHC-DR-β, MHC-DR-associated protein
CD74, tissue factor pathway inhibitor-2, and neuroserpin were much higher in MSC than in
fibroblasts, even in the presence of large interindividual variations. Those of
adrenomedullin, apolipoprotein D, C-type lectin superfamily member-2, collagen type XV α1 …
human bone marrow MSC (11 lines) and human fibroblasts (4 lines) by RT-PCR and real
time PCR. Messenger RNA levels of MHC-DR-α, MHC-DR-β, MHC-DR-associated protein
CD74, tissue factor pathway inhibitor-2, and neuroserpin were much higher in MSC than in
fibroblasts, even in the presence of large interindividual variations. Those of
adrenomedullin, apolipoprotein D, C-type lectin superfamily member-2, collagen type XV α1 …
To characterize mesenchymal stem cells (MSC), we compared gene expression profiles in human bone marrow MSC (11 lines) and human fibroblasts (4 lines) by RT-PCR and real time PCR. Messenger RNA levels of MHC-DR-α, MHC-DR-β, MHC-DR-associated protein CD74, tissue factor pathway inhibitor-2, and neuroserpin were much higher in MSC than in fibroblasts, even in the presence of large interindividual variations. Those of adrenomedullin, apolipoprotein D, C-type lectin superfamily member-2, collagen type XV α1, CUG triplet repeat RNA-binding protein, matrix metalloproteinase-1, protein tyrosine kinase-7, and Sam68-like phosphotyrosine protein/T-STAR were lower in MSC than in fibroblasts. FACS analysis showed that cell surface expression of MHC-DR was also higher in MSC than in fibroblasts. MHC-DR expression decreased after osteogenic differentiation, whereas the expression of adrenomedullin—a potent stimulator of osteoblast activity—along with collagen XV α1 and apolipoprotein D increased after osteogenic differentiation. The marker genes identified in this study should be useful for characterization of MSC both in basic and clinical studies.
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