[HTML][HTML] Topical delivery of siRNA-based spherical nucleic acid nanoparticle conjugates for gene regulation
D Zheng, DA Giljohann, DL Chen… - Proceedings of the …, 2012 - National Acad Sciences
Topical application of nucleic acids offers many potential therapeutic advantages for
suppressing genes in the skin, and potentially for systemic gene delivery. However, the
epidermal barrier typically precludes entry of gene-suppressing therapy unless the barrier is
disrupted. We now show that spherical nucleic acid nanoparticle conjugates (SNA-NCs),
gold cores surrounded by a dense shell of highly oriented, covalently immobilized siRNA,
freely penetrate almost 100% of keratinocytes in vitro, mouse skin, and human epidermis …
suppressing genes in the skin, and potentially for systemic gene delivery. However, the
epidermal barrier typically precludes entry of gene-suppressing therapy unless the barrier is
disrupted. We now show that spherical nucleic acid nanoparticle conjugates (SNA-NCs),
gold cores surrounded by a dense shell of highly oriented, covalently immobilized siRNA,
freely penetrate almost 100% of keratinocytes in vitro, mouse skin, and human epidermis …