[HTML][HTML] Vascular endothelial growth factor induces expression of the antiapoptotic proteins Bcl-2 and A1 in vascular endothelial cells
We examined the role of vascular endothelial growth factor (VEGF) in preventing apoptosis
in primary human umbilical vein endothelial (HUVE) cells. VEGF was capable of preventing
serum starvation-induced apoptosis at concentrations between 10 and 100 ng/ml. The
addition of VEGF to serum-starved HUVE cells led to a 5.2-fold induction of Bcl-2 after 36 h
and to a transient, 2.4-fold induction of A1 after a 7-h incubation, as quantitated by real time
reverse transcriptase-polymerase chain reaction analysis. Western blot analysis …
in primary human umbilical vein endothelial (HUVE) cells. VEGF was capable of preventing
serum starvation-induced apoptosis at concentrations between 10 and 100 ng/ml. The
addition of VEGF to serum-starved HUVE cells led to a 5.2-fold induction of Bcl-2 after 36 h
and to a transient, 2.4-fold induction of A1 after a 7-h incubation, as quantitated by real time
reverse transcriptase-polymerase chain reaction analysis. Western blot analysis …